› 4e09

X-ray diffraction
2.99Å resolution

Structure of ParF-AMPPCP, I422 form

Released:
DOI: 10.2210/pdb4e09/pdb
PDB entry 4e09 coloured by chain, front view.
PDB entry 4e09 coloured by chain, side view.
PDB entry 4e09 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation.
Schumacher MA, Ye Q, Barge MT, Zampini M, Barillà D, Hayes F
J Biol Chem 287 26146-54 (2012)
PMID: 22674577

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B0ZE06 (Residues: 1-206; Coverage: 100%)
Gene names: CG831_004316, DNQ45_07005, EIZ93_19540, HEP30_021835, pOLA52_52, parF
Sequence domains: CobQ/CobB/MinD/ParA nucleotide binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I422
Unit cell:
a: 87.6Å b: 87.6Å c: 150Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.277 0.299
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Surfing biological surfaces: exploiting the nucleoid for partition and transport in bacteria.
Vecchiarelli et al. (2012)
3 more

3 mentions without citation

Methanogen diversity evidenced by molecular characterization of methyl coenzyme M reductase A (mcrA) genes in hydrothermal sediments of the Guaymas Basin.
Dhillon et al. (2005)
2 more