› 4e0j

X-ray diffraction
2.3Å resolution

Protelomerase tela R255A mutant complexed with DNA hairpin product

Released:
DOI: 10.2210/pdb4e0j/pdb
PDB entry 4e0j coloured by chain, front view.
PDB entry 4e0j coloured by chain, side view.
PDB entry 4e0j coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
An enzyme-catalyzed multistep DNA refolding mechanism in hairpin telomere formation.
Shi K, Huang WM, Aihara H
PLoS Biol 11 e1001472 (2013)
PMID: 23382649

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117370 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Telomere resolvase ResT/TelK catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 52.48 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CWV1 (Residues: 103-421; Coverage: 72%)
Gene names: Atu2523, telA
Sequence domains: Telomere resolvase
Structure domains:
DNA (5'-D(*CP*AP*TP*AP*AP*TP*AP*AP*CP*AP*AP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*TP*GP*AP*TP*AP*TP*TP*GP*TP*TP*AP*TP*TP*AP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 117.56Å b: 120.08Å c: 56.76Å
α: 90° β: 111.68° γ: 90°
R-values:
R R work R free
0.208 0.206 0.252
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Telomeres and Their Neighbors.
Jenner et al. (2022)
8 other articles

1 mention without citation

An enzyme-catalyzed multistep DNA refolding mechanism in hairpin telomere formation.
Shi et al. (2013)