› 4e10

X-ray diffraction
2.51Å resolution

Protelomerase tela Y201A covalently complexed with substrate DNA

Released:
DOI: 10.2210/pdb4e10/pdb
PDB entry 4e10 coloured by chain, front view.
PDB entry 4e10 coloured by chain, side view.
PDB entry 4e10 coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
An enzyme-catalyzed multistep DNA refolding mechanism in hairpin telomere formation.
Shi K, Huang WM, Aihara H
PLoS Biol 11 e1001472 (2013)
PMID: 23382649

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117374 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Telomere resolvase ResT/TelK catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 52.56 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CWV1 (Residues: 103-420; Coverage: 72%)
Gene names: Atu2523, telA
Sequence domains: Telomere resolvase
Structure domains:
DNA (5'-D(*CP*AP*TP*AP*AP*(BRU)P*AP*AP*CP*AP*AP*(BRU)P*AP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.15 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*GP*AP*TP*AP*(BRU)P*(BRU)P*GP*(BRU)P*(BRU)P*AP*(BRU)P*(BRU)P*AP*(BRU)P*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.99 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand DT 1 x DT
2 modified residues:
Ligand PTR 1 x PTR
Ligand BRU 10 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 116.34Å b: 119.66Å c: 56.63Å
α: 90° β: 111.3° γ: 90°
R-values:
R R work R free
0.188 0.183 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Telomeres and Their Neighbors.
Jenner et al. (2022)
8 other articles

272 mentions without citation

Human antibodies that neutralize HIV-1: identification, structures, and B cell ontogenies.
Kwong et al. (2012)
271 more