› 4e1f

X-ray diffraction
2.1Å resolution

Structure of a VgrG Vibrio cholerae toxin ACD domain Glu16Gln mutant in complex with ADP and Mn++

Released:
DOI: 10.2210/pdb4e1f/pdb
PDB entry 4e1f coloured by chain, front view.
PDB entry 4e1f coloured by chain, side view.
PDB entry 4e1f coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Structure and functional characterization of the Vibrio cholerae toxin from the VgrG/MARTX family.
Durand E, Audoly G, Derrez E, Spinelli S, Ortiz-Lombardia M, Cascales E, Raoult D, Cambillau C
J. Biol. Chem. (2012)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-101772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin cross-linking toxin VgrG1 Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.74 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0H3AIG7 (Residues: 700-1095; Coverage: 36%)
Gene names: VC0395_A1026, vgrG-1, vgrG1
Sequence domains: Actin cross-linking domain
Structure domains: Actin cross-linking domain

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MN 1 x MN
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P4322
Unit cell:
a: 129.13Å b: 129.13Å c: 77.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.188
Expression system: Escherichia coli BL21

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