› 4e1j

X-ray diffraction
2.33Å resolution

Crystal structure of glycerol kinase in complex with glycerol from Sinorhizobium meliloti 1021

Released:
DOI: 10.2210/pdb4e1j/pdb
PDB entry 4e1j coloured by chain, front view.
PDB entry 4e1j coloured by chain, side view.
PDB entry 4e1j coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Agarwal R, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Siedel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 520 amino acids
Theoretical weight: 57.69 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O86033 (Residues: 1-497; Coverage: 100%)
Gene names: RB1135, SMb21009, glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand NA 2 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 93.22Å b: 101.975Å c: 107.548Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.233 0.23 0.29
Expression system: Escherichia coli BL21(DE3)

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