› 4e1s

X-ray diffraction
1.86Å resolution

X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7

Released:
DOI: 10.2210/pdb4e1s/pdb
PDB entry 4e1s coloured by chain, front view.
PDB entry 4e1s coloured by chain, side view.
PDB entry 4e1s coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis.
Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK
Structure 20 1233-43 (2012)
PMID: 22658748

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intimin Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.79 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43261 (Residues: 208-449; Coverage: 26%)
Gene names: ECs4559, L0025, Z5110, eae, eaeA
Sequence domains: Inverse autotransporter, beta-domain
Structure domains: Inverse autotransporter, beta-domain

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand OLC 10 x OLC
Ligand OLB 3 x OLB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 116.565Å b: 120.248Å c: 39.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Emerging methods in protein co-evolution.
de Juan et al. (2013)
12 more

16 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
15 more