› 4e2b

X-ray diffraction
1.27Å resolution

High resolution crystal structure of the old yellow enzyme from Trypanosoma cruzi

Released:
DOI: 10.2210/pdb4e2b/pdb
PDB entry 4e2b coloured by chain, front view.
PDB entry 4e2b coloured by chain, side view.
PDB entry 4e2b coloured by chain, top view.
Source organism: Trypanosoma cruzi
Entry authors: Murakami MT, Rodrigues NC, Gava LM, Canduri F, Oliva G, Barbosa LRS, Borgers JC

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 42.56 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TJB8 (Residues: 1-379; Coverage: 100%)
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand FMN 1 x FMN
Ligand GOL 3 x GOL
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 57.347Å b: 68.656Å c: 85.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.171
Expression system: Escherichia coli

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