› 4e34

X-ray diffraction
1.4Å resolution

Crystal structure of CFTR Associated Ligand (CAL) PDZ domain bound to iCAL36 (ANSRWPTSII) peptide

Released:
DOI: 10.2210/pdb4e34/pdb
PDB entry 4e34 coloured by chain, front view.
PDB entry 4e34 coloured by chain, side view.
PDB entry 4e34 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stereochemical determinants of C-terminal specificity in PDZ peptide-binding domains: a novel contribution of the carboxylate-binding loop.
Amacher JF, Cushing PR, Bahl CD, Beck T, Madden DR
J Biol Chem 288 5114-26 (2013)
PMID: 23243314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164418 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgi-associated PDZ and coiled-coil motif-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 9.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HD26 (Residues: 284-370; Coverage: 19%)
Gene names: CAL, FIG, GOPC
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
decameric peptide, iCAL36 Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 35.878Å b: 47.709Å c: 97.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.197
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Emerging Themes in PDZ Domain Signaling: Structure, Function, and Inhibition.
Liu et al. (2019)
1 more

10 mentions without citation

Specificity in PDZ-peptide interaction networks: Computational analysis and review.
Amacher et al. (2020)
9 more