› 4e3q

X-ray diffraction
1.9Å resolution

PMP-bound form of Aminotransferase crystal structure from Vibrio fluvialis

Released:
DOI: 10.2210/pdb4e3q/pdb
PDB entry 4e3q coloured by chain, front view.
PDB entry 4e3q coloured by chain, side view.
PDB entry 4e3q coloured by chain, top view.
Source organism: Vibrio fluvialis
Primary publication:
Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin.
Midelfort KS, Kumar R, Han S, Karmilowicz MJ, McConnell K, Gehlhaar DK, Mistry A, Chang JS, Anderson M, Villalobos A, Minshull J, Govindarajan S, Wong JW
Protein Eng Des Sel 26 25-33 (2013)
PMID: 23012440

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-123422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate transaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 473 amino acids
Theoretical weight: 53.06 KDa
Source organism: Vibrio fluvialis
Expression system: Escherichia coli
UniProt:
  • Canonical: F2XBU9 (Residues: 1-453; Coverage: 100%)
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 4 x NA
Ligand SO4 2 x SO4
Ligand BEN 3 x BEN
Ligand PMP 4 x PMP
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 63.071Å b: 162.182Å c: 180.398Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications.
Steffen-Munsberg et al. (2015)
5 more

15 mentions without citation

Structural Consideration of the Working Mechanism of Fold Type I Transaminases From Eubacteria: Overt and Covert Movement.
Kwon et al. (2019)
14 more