› 4e5y

X-ray diffraction
2.37Å resolution

Structure of human FX protein, the key enzyme in the biosynthesis of GDP-L-fucose

Released:
DOI: 10.2210/pdb4e5y/pdb
PDB entry 4e5y coloured by chain, front view.
PDB entry 4e5y coloured by chain, side view.
PDB entry 4e5y coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zhou H, He JH

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-L-fucose synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 35.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13630 (Residues: 1-321; Coverage: 100%)
Gene names: GFUS, SDR4E1, TSTA3
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 4 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 85.772Å b: 136.44Å c: 139.849Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.292
Expression system: Escherichia coli

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