› 4e6w

X-ray diffraction
2.19Å resolution

ClbP in complex with 3-aminophenyl boronic acid

Released:
DOI: 10.2210/pdb4e6w/pdb
PDB entry 4e6w coloured by chain, front view.
PDB entry 4e6w coloured by chain, side view.
PDB entry 4e6w coloured by chain, top view.
Source organism: Escherichia coli O6
Entry authors: Cougnoux A, Delmas J, Bonnet R

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 335 amino acids
Theoretical weight: 36.8 KDa
Source organism: Escherichia coli O6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2V8D3 (Residues: 41-375; Coverage: 71%)
Gene name: c2452
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand APB 3 x APB
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 104.49Å b: 149.36Å c: 88.24Å
α: 90° β: 124.21° γ: 90°
R-values:
R R work R free
0.197 0.195 0.24
Expression system: Escherichia coli BL21(DE3)

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