› 4e70

X-ray diffraction
1.61Å resolution

Crystal Structure Analysis of Coniferyl Alcohol 9-O-Methyltransferase from Linum Nodiflorum in Complex with Coniferyl Alcohol

Released:
DOI: 10.2210/pdb4e70/pdb
PDB entry 4e70 coloured by chain, front view.
PDB entry 4e70 coloured by chain, side view.
PDB entry 4e70 coloured by chain, top view.
Source organism: Linum nodiflorum
Primary publication:
Structural analysis of coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum reveals a novel active-site environment.
Wolters S, Neeb M, Berim A, Schulze Wischeler J, Petersen M, Heine A
Acta Crystallogr D Biol Crystallogr 69 888-900 (2013)
PMID: 23633600

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosyl-L-methionine-dependent coniferyl alcohol 9-O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.43 KDa
Source organism: Linum nodiflorum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6XNE6 (Residues: 1-368; Coverage: 100%)
Gene name: CA9OMT
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand N7I 2 x N7I
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 76.018Å b: 104.451Å c: 109.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Crystal structures of PigF, an O-methyltransferase involved in the prodigiosin synthetic pathway, reveal an induced-fit substrate-recognition mechanism.
Qiu et al. (2022)
1 more

2 mentions without citation

Structural analysis of coniferyl alcohol 9-O-methyltransferase from Linum nodiflorum reveals a novel active-site environment.
Wolters et al. (2013)
1 more