› 4e77

X-ray diffraction
2Å resolution

2.0A Crystal Structure of a Glutamate-1-Semialdehyde Aminotransferase from Yersinia pestis CO92

Released:
DOI: 10.2210/pdb4e77/pdb
PDB entry 4e77 coloured by chain, front view.
PDB entry 4e77 coloured by chain, side view.
PDB entry 4e77 coloured by chain, top view.
Source organism: Yersinia pestis Pestoides F
Entry authors: Brunzelle JS, Wawrzak W, Onopriyenko O, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 46.9 KDa
Source organism: Yersinia pestis Pestoides F
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZBL9 (Residues: 1-426; Coverage: 100%)
Gene names: YPO3389, YP_0296, hemL, y0799
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand NO3 1 x NO3
1 modified residue:
Ligand MSE 17 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 60.54Å b: 60.54Å c: 182.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.197
Expression system: Escherichia coli BL21(DE3)

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