› 4e8f

X-ray diffraction
2.6Å resolution

Structural Basis for the Activity of a Cytoplasmic RNA Terminal U-transferase

Released:
DOI: 10.2210/pdb4e8f/pdb
PDB entry 4e8f coloured by chain, front view.
PDB entry 4e8f coloured by chain, side view.
PDB entry 4e8f coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.
Yates LA, Fleurdépine S, Rissland OS, De Colibus L, Harlos K, Norbury CJ, Gilbert RJC
Nat Struct Mol Biol 19 782-787 (2012)
PMID: 22751018

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase cid1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 46.32 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O13833 (Residues: 1-405; Coverage: 100%)
Gene names: SPAC19D5.03, cid1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 81.15Å b: 101.21Å c: 113.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

The long and short of microRNA.
Yates et al. (2013)
7 more

3 mentions without citation

Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.
Yates et al. (2012)
2 more