› 4e9e

X-ray diffraction
1.9Å resolution

Structure of the glycosylase domain of MBD4

Released:
DOI: 10.2210/pdb4e9e/pdb
PDB entry 4e9e coloured by chain, front view.
PDB entry 4e9e coloured by chain, side view.
PDB entry 4e9e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and structural characterization of the glycosylase domain of MBD4 bound to thymine and 5-hydroxymethyuracil-containing DNA.
Moréra S, Grin I, Vigouroux A, Couvé S, Henriot V, Saparbaev M, Ishchenko AA
Nucleic Acids Res 40 9917-26 (2012)
PMID: 22848106

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-CpG-binding domain protein 4 Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 19.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95243 (Residues: 427-580; Coverage: 27%)
Gene names: MBD4, MED1
Structure domains: Hypothetical protein; domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: R3
Unit cell:
a: 80.97Å b: 80.97Å c: 74.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Base excision repair of oxidative DNA damage: from mechanism to disease.
Whitaker et al. (2017)
18 more

5 mentions without citation

Biochemical and structural characterization of the glycosylase domain of MBD4 bound to thymine and 5-hydroxymethyuracil-containing DNA.
Moréra et al. (2012)
4 more