› 4ea4

X-ray diffraction
2Å resolution

Structure of the glycosylase domain of MBD4 bound to 5hmU-containing DNA

Released:
DOI: 10.2210/pdb4ea4/pdb
PDB entry 4ea4 coloured by chain, front view.
PDB entry 4ea4 coloured by chain, side view.
PDB entry 4ea4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical and structural characterization of the glycosylase domain of MBD4 bound to thymine and 5-hydroxymethyuracil-containing DNA.
Moréra S, Grin I, Vigouroux A, Couvé S, Henriot V, Saparbaev M, Ishchenko AA
Nucleic Acids Res 40 9917-26 (2012)
PMID: 22848106

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117396 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Methyl-CpG-binding domain protein 4 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 18.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95243 (Residues: 427-574; Coverage: 26%)
Gene names: MBD4, MED1
Structure domains: Hypothetical protein; domain 2
DNA (5'-D(*CP*CP*AP*GP*CP*GP*(5HU)P*GP*CP*AP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*CP*TP*GP*CP*GP*CP*GP*CP*TP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.7 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5HU 1 x 5HU

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 40.27Å b: 63.18Å c: 94.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Base excision repair of oxidative DNA damage: from mechanism to disease.
Whitaker et al. (2017)
18 more

2 mentions without citation

Biochemical and structural characterization of the glycosylase domain of MBD4 bound to thymine and 5-hydroxymethyuracil-containing DNA.
Moréra et al. (2012)
1 more