› 4eaf

X-ray diffraction
1.55Å resolution

Thymidine phosphorylase from E.coli

Released:
DOI: 10.2210/pdb4eaf/pdb
PDB entry 4eaf coloured by chain, front view.
PDB entry 4eaf coloured by chain, side view.
PDB entry 4eaf coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Timofeev VI, Abramchik YA, Esipov RS, Kuranova IP

Function and Biology Details

Reaction catalysed: 
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 47.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P07650 (Residues: 2-440; Coverage: 100%)
Gene names: JW4345, b4382, deoA, tpp, ttg
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43212
Unit cell:
a: 129.951Å b: 129.951Å c: 67.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.207
Expression system: Escherichia coli

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