› 4eaw

X-ray diffraction
2Å resolution

HCV NS5B in complex with IDX375

Released:
DOI: 10.2210/pdb4eaw/pdb
PDB entry 4eaw coloured by chain, front view.
PDB entry 4eaw coloured by chain, side view.
PDB entry 4eaw coloured by chain, top view.
Source organism: Hepatitis C virus subtype 1b
Entry authors: Dousson CB, Paparin J-L, Surleraux D, Augustin M, Blaesse M, Hoeppner S, Krapp S, Wenzkowski C

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 562 amino acids
Theoretical weight: 62.58 KDa
Source organism: Hepatitis C virus subtype 1b
Expression system: Escherichia coli
UniProt:
  • Canonical: O92972 (Residues: 2420-2981; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase

Ligands and Environments

4 bound ligands:
Ligand 0NQ 2 x 0NQ
Ligand SO4 8 x SO4
Ligand PEG 3 x PEG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 106.313Å b: 108.028Å c: 133.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.219
Expression system: Escherichia coli

Quick links