› 4eb0

X-ray diffraction
1.5Å resolution

Crystal structure of Leaf-branch compost bacterial cutinase homolog

Released:
DOI: 10.2210/pdb4eb0/pdb
PDB entry 4eb0 coloured by chain, front view.
PDB entry 4eb0 coloured by chain, side view.
PDB entry 4eb0 coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Sulaiman S, You DJ, Eiko K, Koga Y, Kanaya S

Function and Biology Details

Reactions catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leaf-branch compost cutinase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.8 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: G9BY57 (Residues: 36-293; Coverage: 100%)
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand SCN 4 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 40.911Å b: 71.084Å c: 72.726Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.186
Expression system: Escherichia coli

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