› 4eb6

X-ray diffraction
3.47Å resolution

Tubulin-Vinblastine: Stathmin-like complex

Released:
DOI: 10.2210/pdb4eb6/pdb
PDB entry 4eb6 coloured by chain, front view.
PDB entry 4eb6 coloured by chain, side view.
PDB entry 4eb6 coloured by chain, top view.
Source organisms:
Primary publication:
Structural plasticity of tubulin assembly probed by vinca-domain ligands.
Ranaivoson FM, Gigant B, Berritt S, Joullié M, Knossow M
Acta Crystallogr D Biol Crystallogr 68 927-34 (2012)
PMID: 22868758

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-112169 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50.04 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

5 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand SO4 5 x SO4
Ligand GDP 2 x GDP
Ligand VLB 1 x VLB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 64.69Å b: 129.68Å c: 252.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Antitubulin sulfonamides: The successful combination of an established drug class and a multifaceted target.
Vicente-Blázquez et al. (2019)
1 more

11 mentions without citation

Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate.
Trofimova et al. (2018)
10 more