› 4ec5

X-ray diffraction
2.2Å resolution

Crystal structure of the S210C (dimer) mutant from the N-terminal domain of the secretin XcpQ from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb4ec5/pdb
PDB entry 4ec5 coloured by chain, front view.
PDB entry 4ec5 coloured by chain, side view.
PDB entry 4ec5 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
New insights into the assembly of bacterial secretins: structural studies of the periplasmic domain of XcpQ from Pseudomonas aeruginosa.
Van der Meeren R, Wen Y, Van Gelder P, Tommassen J, Devreese B, Savvides SN
J Biol Chem 288 1214-25 (2013)
PMID: 23188826

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Secretin XcpQ Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 26.54 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P35818 (Residues: 35-277; Coverage: 39%)
Gene names: PA3105, xcpQ
Sequence domains:
Structure domains: Ribosomal Protein S8; Chain: A, domain 1

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 120.79Å b: 39.78Å c: 93.9Å
α: 90° β: 99.75° γ: 90°
R-values:
R R work R free
0.214 0.213 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Type II secretion system: a magic beanstalk or a protein escalator.
Nivaskumar et al. (2014)
1 more

1 mention without citation

New insights into the assembly of bacterial secretins: structural studies of the periplasmic domain of XcpQ from Pseudomonas aeruginosa.
Van der Meeren et al. (2013)