› 4ec6

X-ray diffraction
2.5Å resolution

Ntf2-like, potential transfer protein TraM from Gram-positive conjugative plasmid pIP501

Released:
DOI: 10.2210/pdb4ec6/pdb
PDB entry 4ec6 coloured by chain, front view.
PDB entry 4ec6 coloured by chain, side view.
PDB entry 4ec6 coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
The 2.5 Å structure of the enterococcus conjugation protein TraM resembles VirB8 type IV secretion proteins.
Goessweiner-Mohr N, Grumet L, Arends K, Pavkov-Keller T, Gruber CC, Gruber K, Birner-Gruenberger R, Kropec-Huebner A, Huebner J, Grohmann E, Keller W
J Biol Chem 288 2018-28 (2013)
PMID: 23188825

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conjugal transfer protein-like C-terminal domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 161 amino acids
Theoretical weight: 19.02 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8L1C7 (Residues: 190-322; Coverage: 41%)
Sequence domains: Conjugal transfer protein-like, C-terminal domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 39.21Å b: 54.98Å c: 93.47Å
α: 89.91° β: 86.44° γ: 78.63°
R-values:
R R work R free
0.212 0.209 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mechanism and structure of the bacterial type IV secretion systems.
Christie et al. (2014)
7 more

5 mentions without citation

Structural organisation of the type IV secretion systems.
Waksman et al. (2014)
4 more