› 4eca

X-ray diffraction
2.2Å resolution

ASPARAGINASE FROM E. COLI, MUTANT T89V WITH COVALENTLY BOUND ASPARTATE

Released:
DOI: 10.2210/pdb4eca/pdb
PDB entry 4eca coloured by chain, front view.
PDB entry 4eca coloured by chain, side view.
PDB entry 4eca coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
A covalently bound catalytic intermediate in Escherichia coli asparaginase: crystal structure of a Thr-89-Val mutant.
Palm GJ, Lubkowski J, Derst C, Schleper S, Röhm KH, Wlodawer A
FEBS Lett 390 211-6 (1996)
PMID: 8706862

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 326 amino acids
Theoretical weight: 34.74 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00805 (Residues: 23-348; Coverage: 100%)
Gene names: JW2924, ansB, b2957
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand AEI 4 x AEI

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 95Å b: 126.2Å c: 155.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Escherichia coli

Quick links

Citations

6 review citations

From one amino acid to another: tRNA-dependent amino acid biosynthesis.
Sheppard et al. (2008)
5 more

4 mentions without citation

Improving the Treatment of Acute Lymphoblastic Leukemia.
Radadiya et al. (2020)
3 more