› 4ecd

X-ray diffraction
2.5Å resolution

2.5 Angstrom Resolution Crystal Structure of Bifidobacterium longum Chorismate Synthase

Released:
DOI: 10.2210/pdb4ecd/pdb
PDB entry 4ecd coloured by chain, front view.
PDB entry 4ecd coloured by chain, side view.
PDB entry 4ecd coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Entry authors: Light SH, Minasov G, Krishna SN, Shuvalova L, Kwon K, Lavie A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 42.65 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B7GS97 (Residues: 1-395; Coverage: 100%)
Gene names: BLIJ_1653, Blon_1598, aroC
Sequence domains: Chorismate synthase
Structure domains: Chorismate synthase AroC

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P42212
Unit cell:
a: 123.944Å b: 123.944Å c: 97.884Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.253
Expression system: Escherichia coli BL21

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