› 4ed5

X-ray diffraction
2Å resolution

Crystal structure of the two N-terminal RRM domains of HuR complexed with RNA

Released:
DOI: 10.2210/pdb4ed5/pdb
PDB entry 4ed5 coloured by chain, front view.
PDB entry 4ed5 coloured by chain, side view.
PDB entry 4ed5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the ARE-binding domains of Hu antigen R (HuR) undergoes conformational changes during RNA binding.
Wang H, Zeng F, Liu Q, Liu H, Liu Z, Niu L, Teng M, Li X
Acta Crystallogr D Biol Crystallogr 69 373-80 (2013)
PMID: 23519412

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172380 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ELAV-like protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15717 (Residues: 18-186; Coverage: 52%)
Gene names: ELAVL1, HUR
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*A*UP*UP*UP*UP*UP*AP*UP*UP*UP*U)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 nucleotides
Theoretical weight: 3.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GOL 7 x GOL
Ligand M2M 1 x M2M
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 136.77Å b: 62.75Å c: 53.29Å
α: 90° β: 111.89° γ: 90°
R-values:
R R work R free
0.211 0.209 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Musashi RNA-Binding Proteins as Cancer Drivers and Novel Therapeutic Targets.
Kudinov et al. (2017)
11 more

23 mentions without citation

Hu Antigen R (HuR) Protein Structure, Function and Regulation in Hepatobiliary Tumors.
Lachiondo-Ortega et al. (2022)
22 more