› 4efh

X-ray diffraction
2.48Å resolution

Acanthamoeba Actin complex with Spir domain D

Released:
DOI: 10.2210/pdb4efh/pdb
PDB entry 4efh coloured by chain, front view.
PDB entry 4efh coloured by chain, side view.
PDB entry 4efh coloured by chain, top view.
Source organisms:
Primary publication:
Multiple forms of Spire-actin complexes and their functional consequences.
Chen CK, Sawaya MR, Phillips ML, Reisler E, Quinlan ME
J Biol Chem 287 10684-10692 (2012)
PMID: 22334675

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135934 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-1 Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.72 KDa
Source organism: Acanthamoeba castellanii
UniProt:
  • Canonical: P02578 (Residues: 1-375; Coverage: 100%)
Sequence domains: Actin
Structure domains:
Protein spire Chain: B
Molecule details ›
Chain: B
Length: 66 amino acids
Theoretical weight: 7.32 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U1K1 (Residues: 428-485; Coverage: 6%)
Gene names: CG10076, p150-Spir, spir

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 53.006Å b: 71.791Å c: 126.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.207
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Guardians of the actin monomer.
Xue et al. (2013)
2 more

4 mentions without citation

Filament assembly by Spire: key residues and concerted actin binding.
Rasson et al. (2015)
3 more