› 4efj

X-ray diffraction
2.8Å resolution

Crystal structure of I-GzeII LAGLIDADG homing endonuclease in complex with DNA target site

Released:
DOI: 10.2210/pdb4efj/pdb
PDB entry 4efj coloured by chain, front view.
PDB entry 4efj coloured by chain, side view.
PDB entry 4efj coloured by chain, top view.
Source organism: Fusarium graminearum
Entry author: Kulshina N

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107637 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 300 amino acids
Theoretical weight: 34.78 KDa
Source organism: Fusarium graminearum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5J036 (Residues: 50-348; Coverage: 86%)
Gene name: nad2
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA target site top strand Chains: C, E
Molecule details ›
Chains: C, E
Length: 27 nucleotides
Theoretical weight: 8.45 KDa
Source organism: Fusarium graminearum
Expression system: Not provided
DNA target site bottom strand Chains: D, F
Molecule details ›
Chains: D, F
Length: 27 nucleotides
Theoretical weight: 8.15 KDa
Source organism: Fusarium graminearum
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 125.827Å b: 61.178Å c: 128.877Å
α: 90° β: 116.3° γ: 90°
R-values:
R R work R free
0.188 0.183 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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