› 4ehb

X-ray diffraction
1.85Å resolution

Crystal structure of the CFTR inhibitory factor Cif with the D129S mutation bound to epoxyhexane

Released:
DOI: 10.2210/pdb4ehb/pdb
PDB entry 4ehb coloured by chain, front view.
PDB entry 4ehb coloured by chain, side view.
PDB entry 4ehb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif.
Bahl CD, Hvorecny KL, Morisseau C, Gerber SA, Madden DR
Biochemistry 55 788-97 (2016)
PMID: 26752215

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 34.14 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZD27 (Residues: 25-319; Coverage: 100%)
Gene name: PA14_26090
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 0PZ 4 x 0PZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 167.43Å b: 83.92Å c: 88.74Å
α: 90° β: 100.31° γ: 90°
R-values:
R R work R free
0.179 0.177 0.214
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Pseudomonas aeruginosa sabotages the generation of host proresolving lipid mediators.
Flitter et al. (2017)
6 more

2 mentions without citation

Root angle in maize influences nitrogen capture and is regulated by calcineurin B-like protein (CBL)-interacting serine/threonine-protein kinase 15 (ZmCIPK15).
Schneider et al. (2022)
1 more