› 4eip

X-ray diffraction
2.33Å resolution

Native and K252c bound RebC-10x

Released:
DOI: 10.2210/pdb4eip/pdb
Model geometry
Fit model/data
PDB entry 4eip coloured by chain, front view.
PDB entry 4eip coloured by chain, side view.
PDB entry 4eip coloured by chain, top view.
Source organism: Lentzea aerocolonigenes
Primary publication:
An unusual role for a mobile flavin in StaC-like indolocarbazole biosynthetic enzymes.
Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan CL
Chem Biol 19 855-65 (2012)
PMID: 22840773

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 59.68 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KI25 (Residues: 1-529; Coverage: 100%)
Gene names: rbmD, rebC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand K2C 1 x K2C
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 64.305Å b: 78.593Å c: 125.723Å
α: 90° β: 99.87° γ: 90°
R-values:
R R work R free
0.216 0.213 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Flavin dependent monooxygenases.
Huijbers et al. (2014)
6 more

4 mentions without citation

An unusual role for a mobile flavin in StaC-like indolocarbazole biosynthetic enzymes.
Goldman et al. (2012)
3 more