› 4el8

X-ray diffraction
2.45Å resolution

The unliganded structure of C.bescii CelA GH48 module

Released:
DOI: 10.2210/pdb4el8/pdb
PDB entry 4el8 coloured by chain, front view.
PDB entry 4el8 coloured by chain, side view.
PDB entry 4el8 coloured by chain, top view.
Source organism: Caldicellulosiruptor bescii DSM 6725
Primary publication:
Revealing nature's cellulase diversity: the digestion mechanism of Caldicellulosiruptor bescii CelA.
Brunecky R, Alahuhta M, Xu Q, Donohoe BS, Crowley MF, Kataeva IA, Yang SJ, Resch MG, Adams MW, Lunin VV, Himmel ME, Bomble YJ
Science 342 1513-6 (2013)
PMID: 24357319

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 634 amino acids
Theoretical weight: 72.24 KDa
Source organism: Caldicellulosiruptor bescii DSM 6725
Expression system: Escherichia coli
UniProt:
  • Canonical: B9MKU7 (Residues: 1126-1759; Coverage: 36%)
Gene name: Athe_1867
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand SO4 1 x SO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 116.3Å b: 57.666Å c: 106.06Å
α: 90° β: 102.3° γ: 90°
R-values:
R R work R free
0.199 0.195 0.258
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Lignocellulose degradation mechanisms across the Tree of Life.
Cragg et al. (2015)
18 more

3 mentions without citation

Sequence, structure, and evolution of cellulases in glycoside hydrolase family 48.
Sukharnikov et al. (2012)
2 more