› 4eme

X-ray diffraction
2.6Å resolution

X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase

Released:
DOI: 10.2210/pdb4eme/pdb
PDB entry 4eme coloured by chain, front view.
PDB entry 4eme coloured by chain, side view.
PDB entry 4eme coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase PfM18AAP.
Sivaraman KK, Oellig CA, Huynh K, Atkinson SC, Poreba M, Perugini MA, Trenholme KR, Gardiner DL, Salvesen G, Drag M, Dalton JP, Whisstock JC, McGowan S
J Mol Biol 422 495-507 (2012)
PMID: 22709581

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartyl aminopeptidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 571 amino acids
Theoretical weight: 65.85 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I2J3 (Residues: 2-570; Coverage: 100%)
Gene names: DAP, M18AAP, PF3D7_0932300, PFI1570c
Sequence domains: Aminopeptidase I zinc metalloprotease (M18)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P213
Unit cell:
a: 200.384Å b: 200.384Å c: 200.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.196
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Working in concert: the metalloaminopeptidases from Plasmodium falciparum.
McGowan S. (2013)
2 more

5 mentions without citation

Ellagic Acid, Kaempferol, and Quercetin from Acacia nilotica: Promising Combined Drug With Multiple Mechanisms of Action.
Al-Nour et al. (2019)
4 more