› 4emg

X-ray diffraction
2.7Å resolution

Crystal structure of SpLsm3

Released:
DOI: 10.2210/pdb4emg/pdb
PDB entry 4emg coloured by chain, front view.
PDB entry 4emg coloured by chain, side view.
PDB entry 4emg coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Crystal structures of Lsm3, Lsm4 and Lsm5/6/7 from Schizosaccharomyces pombe.
Wu D, Jiang S, Bowler MW, Song H
PLoS One 7 e36768 (2012)
PMID: 22615807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable U6 snRNA-associated Sm-like protein LSm3 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 93 amino acids
Theoretical weight: 10.82 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y7M4 (Residues: 1-93; Coverage: 100%)
Gene names: SPBC9B6.05c, lsm3
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 101.43Å b: 101.7Å c: 143.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.277
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural and functional control of the eukaryotic mRNA decapping machinery.
Arribas-Layton et al. (2013)
2 more

2 mentions without citation

Crystal structures of Lsm3, Lsm4 and Lsm5/6/7 from Schizosaccharomyces pombe.
Wu et al. (2012)
1 more