› 4en1

X-ray diffraction
1.62Å resolution

The 1.62A structure of a FRET-optimized Cerulean Fluorescent Protein

Released:
DOI: 10.2210/pdb4en1/pdb
PDB entry 4en1 coloured by chain, front view.
PDB entry 4en1 coloured by chain, side view.
PDB entry 4en1 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
The 1.6 Å resolution structure of a FRET-optimized Cerulean fluorescent protein.
Watkins JL, Kim H, Markwardt ML, Chen L, Fromme R, Rizzo MA, Wachter RM
Acta Crystallogr D Biol Crystallogr 69 767-73 (2013)
PMID: 23633585

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.59 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

4 bound ligands:
Ligand GOL 10 x GOL
Ligand PEG 3 x PEG
Ligand ACT 1 x ACT
Ligand SO4 1 x SO4
1 modified residue:
Ligand SWG 2 x SWG

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 79.41Å b: 88.726Å c: 94.738Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.205
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Conformational analysis of misfolded protein aggregation by FRET and live-cell imaging techniques.
Kitamura et al. (2015)
1 other article

2 mentions without citation

Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding.
Liu et al. (2017)
1 more