› 4ene

X-ray diffraction
2.4Å resolution

Structure of the N- and C-terminal trimmed ClC-ec1 Cl-/H+ antiporter and Fab Complex

Released:
DOI: 10.2210/pdb4ene/pdb
PDB entry 4ene coloured by chain, front view.
PDB entry 4ene coloured by chain, side view.
PDB entry 4ene coloured by chain, top view.
Source organisms:
Primary publication:
Intracellular proton access in a Cl(-)/H(+) antiporter.
Lim HH, Shane T, Miller C
PLoS Biol 10 e1001441 (2012)
PMID: 23239938

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211180 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 47.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 17-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
heavy chain of Fab fragment Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
light chain of Fab fragment Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand CL 5 x CL
Ligand DMU 6 x DMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 232.26Å b: 97.52Å c: 173.96Å
α: 90° β: 132.87° γ: 90°
R-values:
R R work R free
0.241 0.239 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Mus musculus

Quick links

Citations

5 review citations

CLC channel function and dysfunction in health and disease.
Stölting et al. (2014)
4 more

6 mentions without citation

Improving B-cell epitope prediction and its application to global antibody-antigen docking.
Krawczyk et al. (2014)
5 more