› 4enm

X-ray diffraction
2.84Å resolution

Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-benzylguanine

Released:
DOI: 10.2210/pdb4enm/pdb
PDB entry 4enm coloured by chain, front view.
PDB entry 4enm coloured by chain, side view.
PDB entry 4enm coloured by chain, top view.
Source organisms:
Primary publication:
Atl1 regulates choice between global genome and transcription-coupled repair of O(6)-alkylguanines.
Latypov VF, Tubbs JL, Watson AJ, Marriott AS, McGown G, Thorncroft M, Wilkinson OJ, Senthong P, Butt A, Arvai AS, Millington CL, Povey AC, Williams DM, Santibanez-Koref MF, Tainer JA, Margison GP
Mol Cell 47 50-60 (2012)
PMID: 22658721

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117428 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Alkyltransferase-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.66 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UTN9 (Residues: 1-108; Coverage: 100%)
Gene names: SPAC1250.04c, atl1
Sequence domains: 6-O-methylguanine DNA methyltransferase, DNA binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*CP*CP*AP*TP*GP*(BZG)P*CP*TP*AP*GP*TP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.08 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*TP*AP*CP*TP*AP*GP*CP*CP*AP*TP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6122
Unit cell:
a: 59.9Å b: 59.9Å c: 237.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.173 0.253
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Nucleotide excision repair in eukaryotes.
Schärer OD. (2013)
8 more

1 mention without citation

Atl1 regulates choice between global genome and transcription-coupled repair of O(6)-alkylguanines.
Latypov et al. (2012)