› 4eo4

X-ray diffraction
2.87Å resolution

Crystal structure of the yeast mitochondrial threonyl-tRNA synthetase (MST1) in complex with seryl sulfamoyl adenylate

Released:
DOI: 10.2210/pdb4eo4/pdb
PDB entry 4eo4 coloured by chain, front view.
PDB entry 4eo4 coloured by chain, side view.
PDB entry 4eo4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The mechanism of pre-transfer editing in yeast mitochondrial threonyl-tRNA synthetase.
Ling J, Peterson KM, Simonovic I, Söll D, Simonovic M
J Biol Chem 287 28518-25 (2012)
PMID: 22773845

Function and Biology Details

Reaction catalysed: 
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine--tRNA ligase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 460 amino acids
Theoretical weight: 53.47 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P07236 (Residues: 26-462; Coverage: 95%)
Gene names: MST1, YKL194C
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SSA 8 x SSA
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 213.233Å b: 107.498Å c: 153.391Å
α: 90° β: 130.05° γ: 90°
R-values:
R R work R free
0.178 0.175 0.229
Expression system: Escherichia coli

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Citations

5 review citations

Mistranslation of the genetic code.
Moghal et al. (2014)
4 more

2 mentions without citation

The mechanism of pre-transfer editing in yeast mitochondrial threonyl-tRNA synthetase.
Ling et al. (2012)
1 more