› 4eoc

X-ray diffraction
1.49Å resolution

Crystal structure of h74q Synechocystis sp. PCYA

Released:
DOI: 10.2210/pdb4eoc/pdb
PDB entry 4eoc coloured by chain, front view.
PDB entry 4eoc coloured by chain, side view.
PDB entry 4eoc coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Entry authors: Kabasakal BV, Fisher AJ

Function and Biology Details

Reaction catalysed: 
(3Z)-phycocyanobilin + 4 oxidized ferredoxin = biliverdin IX-alpha + 4 reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phycocyanobilin:ferredoxin oxidoreductase Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.2 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q55891 (Residues: 8-246; Coverage: 96%)
Gene names: pcyA, slr0116
Sequence domains: Ferredoxin-dependent bilin reductase
Structure domains: oxygen-dependent coproporphyrinogen oxidase

Ligands and Environments

2 bound ligands:
Ligand BLA 1 x BLA
Ligand CL 2 x CL
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 70.4Å b: 94.88Å c: 42.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.207
Expression system: Escherichia coli BL21

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