› 4epa

X-ray diffraction
3.2Å resolution

The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis

Released:
DOI: 10.2210/pdb4epa/pdb
PDB entry 4epa coloured by chain, front view.
PDB entry 4epa coloured by chain, side view.
PDB entry 4epa coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Structural engineering of a phage lysin that targets gram-negative pathogens.
Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK
Proc Natl Acad Sci U S A 109 9857-62 (2012)
PMID: 22679291

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticin receptor Chain: A
Molecule details ›
Chain: A
Length: 655 amino acids
Theoretical weight: 72.65 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46359 (Residues: 23-673; Coverage: 100%)
Gene names: YPO1906, YP_1649, fyuA, psn, y2404
Sequence domains:
Structure domains: TonB-dependent receptor, beta-barrel domain

Ligands and Environments

1 bound ligand:
Ligand LDA 2 x LDA
1 modified residue:
Ligand MSE 17 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4122
Unit cell:
a: 181.179Å b: 181.179Å c: 319.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.244
Expression system: Escherichia coli

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Citations

33 review citations

Iron, copper, zinc, and manganese transport and regulation in pathogenic Enterobacteria: correlations between strains, site of infection and the relative importance of the different metal transport systems for virulence.
Porcheron et al. (2013)
32 more

4 mentions without citation

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
3 more