› 4eqb

X-ray diffraction
1.5Å resolution

1.5 Angstrom Crystal Structure of Spermidine/Putrescine ABC Transporter Substrate-Binding Protein PotD from Streptococcus pneumoniae strain Canada MDR_19A in Complex with Calcium and HEPES

Released:
DOI: 10.2210/pdb4eqb/pdb
PDB entry 4eqb coloured by chain, front view.
PDB entry 4eqb coloured by chain, side view.
PDB entry 4eqb coloured by chain, top view.
Source organism: Streptococcus pneumoniae TCH8431/19A
Entry authors: Minasov G, Wawrzak Z, Stogios PJ, Kudritska M, Yim V, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spermidine/putrescine ABC superfamily ATP binding cassette transporter, binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 38.15 KDa
Source organism: Streptococcus pneumoniae TCH8431/19A
Expression system: Escherichia coli BL21
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand CA 8 x CA
Ligand CL 2 x CL
Ligand EPE 2 x EPE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 45.635Å b: 54.347Å c: 76.704Å
α: 75.83° β: 80.87° γ: 77.56°
R-values:
R R work R free
0.157 0.155 0.186
Expression system: Escherichia coli BL21

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