› 4erd

X-ray diffraction
2.59Å resolution

Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65 in complex with stem IV of telomerase RNA

Released:
DOI: 10.2210/pdb4erd/pdb
PDB entry 4erd coloured by chain, front view.
PDB entry 4erd coloured by chain, side view.
PDB entry 4erd coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65.
Singh M, Wang Z, Koo BK, Patel A, Cascio D, Collins K, Feigon J
Mol Cell 47 16-26 (2012)
PMID: 22705372

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196798 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
La-related protein 7 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 15.79 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W7X6T2 (Residues: 375-542; Coverage: 22%)
Gene names: TAP65, TTHERM_000318539
Structure domains: Alpha-Beta Plaits
5'-R(P*GP*GP*UP*CP*GP*AP*CP*AP*UP*CP*UP*UP*CP*GP*GP*AP*UP*GP*GP*AP*CP*C)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 7.05 KDa
Source organism: Tetrahymena thermophila
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 88.83Å b: 88.83Å c: 119.21Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.22 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

18 review citations

Biogenesis of telomerase ribonucleoproteins.
Egan et al. (2012)
17 more

22 mentions without citation

Telomerase structure.
Sandin et al. (2014)
21 more