› 4ers

X-ray diffraction
2.64Å resolution

A Molecular Basis for Negative Regulation of the Glucagon Receptor

Released:
DOI: 10.2210/pdb4ers/pdb
PDB entry 4ers coloured by chain, front view.
PDB entry 4ers coloured by chain, side view.
PDB entry 4ers coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for negative regulation of the glucagon receptor.
Koth CM, Murray JM, Mukund S, Madjidi A, Minn A, Clarke HJ, Wong T, Chiang V, Luis E, Estevez A, Rondon J, Zhang Y, Hötzel I, Allan BB
Proc Natl Acad Sci U S A 109 14393-8 (2012)
PMID: 22908259

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211261 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Glucagon receptor Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 11.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P47871 (Residues: 28-123; Coverage: 21%)
Gene name: GCGR
Sequence domains: Hormone receptor domain
Structure domains: GPCR, family 2, extracellular hormone receptor domain

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 46.41Å b: 62.25Å c: 104.94Å
α: 90° β: 93.91° γ: 90°
R-values:
R R work R free
0.234 0.231 0.278
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

21 review citations

Regulation of the oncoprotein Smoothened by small molecules.
Sharpe et al. (2015)
20 more

20 mentions without citation

Insights into the structure of class B GPCRs.
Hollenstein et al. (2014)
19 more