› 4es6

X-ray diffraction
2.22Å resolution

Crystal structure of HemD (PA5259) from Pseudomonas aeruginosa (PAO1) at 2.22 A resolution

Released:
DOI: 10.2210/pdb4es6/pdb
PDB entry 4es6 coloured by chain, front view.
PDB entry 4es6 coloured by chain, side view.
PDB entry 4es6 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.
Moynie L, Schnell R, McMahon SA, Sandalova T, Boulkerou WA, Schmidberger JW, Alphey M, Cukier C, Duthie F, Kopec J, Liu H, Jacewicz A, Hunter WN, Naismith JH, Schneider G
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 25-34 (2013)
PMID: 23295481

Function and Biology Details

Reaction catalysed: 
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uroporphyrinogen-III synthase Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.59 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48246 (Residues: 1-251; Coverage: 100%)
Gene names: PA5259, hemD
Sequence domains: Uroporphyrinogen-III synthase HemD
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 132.07Å b: 132.07Å c: 42.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
3 more

2 mentions without citation

The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.
Moynie et al. (2013)
1 more