› 4eus

X-ray diffraction
1.65Å resolution

Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol

Released:
DOI: 10.2210/pdb4eus/pdb
PDB entry 4eus coloured by chain, front view.
PDB entry 4eus coloured by chain, side view.
PDB entry 4eus coloured by chain, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif.
Bahl CD, Hvorecny KL, Morisseau C, Gerber SA, Madden DR
Biochemistry 55 788-97 (2016)
PMID: 26752215

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 301 amino acids
Theoretical weight: 34.16 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZD27 (Residues: 25-319; Coverage: 100%)
Gene name: PA14_26090
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 0RE 2 x 0RE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 169.768Å b: 84.249Å c: 90.132Å
α: 90° β: 100.31° γ: 90°
R-values:
R R work R free
0.17 0.169 0.197
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Pseudomonas aeruginosa sabotages the generation of host proresolving lipid mediators.
Flitter et al. (2017)
6 more

1 mention without citation

Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif.
Bahl et al. (2016)