› 4evr

X-ray diffraction
1.84Å resolution

Crystal structure of ABC transporter from R. palustris - solute binding protein (RPA0668) in complex with benzoate

Released:
DOI: 10.2210/pdb4evr/pdb
PDB entry 4evr coloured by chain, front view.
PDB entry 4evr coloured by chain, side view.
PDB entry 4evr coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Primary publication:
Characterization of transport proteins for aromatic compounds derived from lignin: benzoate derivative binding proteins.
Michalska K, Chang C, Mack JC, Zerbs S, Joachimiak A, Collart FR
J Mol Biol 423 555-75 (2012)
PMID: 22925578

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124377 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 39.24 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XCQ4 (Residues: 21-392; Coverage: 95%)
Gene names: RPA0668, TX73_003460, hbaE
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand BEZ 1 x BEZ
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 42.306Å b: 57.778Å c: 133.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Opportunities and challenges in biological lignin valorization.
Beckham et al. (2016)
3 more

1 mention without citation

Characterization of transport proteins for aromatic compounds derived from lignin: benzoate derivative binding proteins.
Michalska et al. (2012)