› 4ewp

X-ray diffraction
2.2Å resolution

Crystal structure of FabH from Micrococcus luteus

Released:
DOI: 10.2210/pdb4ewp/pdb
PDB entry 4ewp coloured by chain, front view.
PDB entry 4ewp coloured by chain, side view.
PDB entry 4ewp coloured by chain, top view.
Source organism: Micrococcus luteus NCTC 2665
Primary publication:
Structure of FabH and factors affecting the distribution of branched fatty acids in Micrococcus luteus.
Pereira JH, Goh EB, Keasling JD, Beller HR, Adams PD
Acta Crystallogr D Biol Crystallogr 68 1320-8 (2012)
PMID: 22993086

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-ketoacyl-[acyl-carrier-protein] synthase III Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 350 amino acids
Theoretical weight: 36.78 KDa
Source organism: Micrococcus luteus NCTC 2665
Expression system: Escherichia coli
UniProt:
  • Canonical: C5CAR9 (Residues: 1-350; Coverage: 100%)
Gene names: Mlut_09310, NCTC2665_01950, fabH, fabH_1
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 80.14Å b: 85.026Å c: 96.916Å
α: 69.82° β: 71.37° γ: 85.66°
R-values:
R R work R free
0.169 0.169 0.21
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Recent advances in inhibitors of bacterial fatty acid synthesis type II (FASII) system enzymes as potential antibacterial agents.
Wang et al. (2013)
3 other articles

2 mentions without citation

Structure of FabH and factors affecting the distribution of branched fatty acids in Micrococcus luteus.
Pereira et al. (2012)
1 more