› 4eyt

X-ray diffraction
2.5Å resolution

Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65

Released:
DOI: 10.2210/pdb4eyt/pdb
PDB entry 4eyt coloured by chain, front view.
PDB entry 4eyt coloured by chain, side view.
PDB entry 4eyt coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65.
Singh M, Wang Z, Koo BK, Patel A, Cascio D, Collins K, Feigon J
Mol Cell 47 16-26 (2012)
PMID: 22705372

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
La-related protein 7 homolog Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 129 amino acids
Theoretical weight: 15.65 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W7X6T2 (Residues: 375-542; Coverage: 22%)
Gene names: TAP65, TTHERM_000318539

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 156.19Å b: 91.9Å c: 82.83Å
α: 90° β: 107.36° γ: 90°
R-values:
R R work R free
0.227 0.225 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Biogenesis of telomerase ribonucleoproteins.
Egan et al. (2012)
22 more

7 mentions without citation

The La and related RNA-binding proteins (LARPs): structures, functions, and evolving perspectives.
Maraia et al. (2017)
6 more