› 4ezd

X-ray diffraction
2.5Å resolution

Crystal Structure of the UT-B Urea Transporter from Bos Taurus Bound to Selenourea

Released:
DOI: 10.2210/pdb4ezd/pdb
PDB entry 4ezd coloured by chain, front view.
PDB entry 4ezd coloured by chain, side view.
PDB entry 4ezd coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure and permeation mechanism of a mammalian urea transporter.
Levin EJ, Cao Y, Enkavi G, Quick M, Pan Y, Tajkhorshid E, Zhou M
Proc Natl Acad Sci U S A 109 11194-9 (2012)
PMID: 22733730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urea transporter 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 384 amino acids
Theoretical weight: 41.5 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5QF96 (Residues: 1-384; Coverage: 100%)
Gene names: SLC14A1, UT-B
Sequence domains: Urea transporter
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

4 bound ligands:
Ligand SEY 6 x SEY
Ligand BGC 8 x BGC
Ligand BOG 10 x BOG
Ligand SPL 3 x SPL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 75.002Å b: 106.227Å c: 105.816Å
α: 90° β: 98.18° γ: 90°
R-values:
R R work R free
0.199 0.197 0.228
Expression system: Spodoptera frugiperda

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Citations

8 review citations

Water in Nanopores and Biological Channels: A Molecular Simulation Perspective.
Lynch et al. (2020)
7 more

11 mentions without citation

The recombinant expression systems for structure determination of eukaryotic membrane proteins.
He et al. (2014)
10 more