› 4eze

X-ray diffraction
2.27Å resolution

Crystal structure of had family hydrolase t0658 from Salmonella enterica subsp. enterica serovar Typhi (Target EFI-501419)

Released:
DOI: 10.2210/pdb4eze/pdb
PDB entry 4eze coloured by chain, front view.
PDB entry 4eze coloured by chain, side view.
PDB entry 4eze coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhi str. Ty2
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Allen KN, Dunaway-Mariano D, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.78 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhi str. Ty2
Expression system: Not provided
UniProt:
  • Canonical: Q8Z598 (Residues: 1-295; Coverage: 100%)
Gene names: CAJ76_02920, CAJ80_11385, CAJ85_15160, CAL67_04850, CB224_02615, CC884_01100, CC907_16695, CC972_08415, D4Z37_02845, D6Q30_07620, DKA88_09710, DNM71_07475, DNV09_18430, DS261_02925, DUQ91_02925, DUV11_04360, DVG36_02275, EBC38_02870, EID90_18470, ELQ62_13415, EU445_02875, EVI00_02335, F9R11_02310, F9Y21_02310, FI137_02165, G2227_02235, G2244_02865, G3982_001569, G3V49_000593, G4I71_002611, G4L28_001453, G4P30_002393, G4Y41_001641, G4Y55_000445, G9264_001204, G9C68_000445, G9X37_000425, GDI29_02310, GND71_000575, K3U68_03330, STY2431, ZZ17_11220, serB, t0658
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 117.634Å b: 117.634Å c: 118.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.246
Expression system: Not provided

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