› 4ezs

X-ray diffraction
1.9Å resolution

Crystal structure of the substrate binding domain of E.coli DnaK in complex with metchnikowin (residues 20 to 26)

Released:
DOI: 10.2210/pdb4ezs/pdb
PDB entry 4ezs coloured by chain, front view.
PDB entry 4ezs coloured by chain, side view.
PDB entry 4ezs coloured by chain, top view.
Source organisms:
Entry authors: Zahn M, Straeter N

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141463 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaK Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y8 (Residues: 389-607; Coverage: 34%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein
Structure domains:
Metchnikowin Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 963 Da
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: Q24395 (Residues: 46-52; Coverage: 25%)
Gene names: CG8175, Mtk

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I222
Unit cell:
a: 94.437Å b: 116.905Å c: 36.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.256
Expression systems:
  • Escherichia coli
  • Not provided

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