› 4f07

X-ray diffraction
2.3Å resolution

Structure of the Styrene Monooxygenase Flavin Reductase (SMOB) from Pseudomonas putida S12

Released:
DOI: 10.2210/pdb4f07/pdb
PDB entry 4f07 coloured by chain, front view.
PDB entry 4f07 coloured by chain, side view.
PDB entry 4f07 coloured by chain, top view.
Source organism: Pseudomonas sp. Y2
Primary publication:
Structure and mechanism of styrene monooxygenase reductase: new insight into the FAD-transfer reaction.
Morrison E, Kantz A, Gassner GT, Sazinsky MH
Biochemistry 52 6063-75 (2013)
PMID: 23909369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin reductase like domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 190 amino acids
Theoretical weight: 20.55 KDa
Source organism: Pseudomonas sp. Y2
Expression system: Escherichia coli
UniProt:
  • Canonical: O33495 (Residues: 1-170; Coverage: 100%)
Gene name: styB
Sequence domains: Flavin reductase like domain
Structure domains: Electron Transport, Fmn-binding Protein; Chain A

Ligands and Environments

3 bound ligands:
Ligand FAD 12 x FAD
Ligand NO3 12 x NO3
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P32
Unit cell:
a: 130.031Å b: 130.031Å c: 95.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.185 0.248
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Flavin dependent monooxygenases.
Huijbers et al. (2014)
3 more

3 mentions without citation

Two-Component FAD-Dependent Monooxygenases: Current Knowledge and Biotechnological Opportunities.
Heine et al. (2018)
2 more